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Simultaneous determination of water‐soluble whitening ingredients and adenosine in different cosmetic formulations by high‐performance liquid chromatography coupled with photodiode array detection
Author(s) -
Jeon J.S.,
Kim H.T.,
Kim M.G.,
Oh M.S.,
Hong S.R.,
Yoon M.H.,
Cho S.M.,
Shin H.C.,
Shim J.H.,
Ramadan A.,
Abd ElAty A. M.
Publication year - 2016
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/ics.12292
Subject(s) - chemistry , chromatography , high performance liquid chromatography , skin whitening , cosmetics , phosphoric acid , niacinamide , hydroquinone , detection limit , active ingredient , organic chemistry , nicotinamide , bioinformatics , biology , enzyme
Objective The Korean Cosmetic Act regulates the use of functional cosmetics) by the law. Four functional cosmetic groups, whitening, anti‐wrinkle, UV protection and combination of whitening and anti‐wrinkle, were categorized according to the Korean Cosmetic Act and Functional Cosmetics Codex. In this study, high‐performance liquid chromatography ( HPLC ) coupled with photodiode array detection ( DAD ) was employed for the simultaneous detection of arbutin (and its decomposition product, hydroquinone), niacinamide, ascorbyl glucoside, ethyl ascorbyl ether and adenosine in functional cosmetic products such as creams, emulsions and lotions. Methods Separation by HPLC ‐ DAD was conducted using a C18 column with a gradient elution of 5 m m KH 2 PO 4 buffer (containing 0.1% phosphoric acid) and methanol (containing 0.1% phosphoric acid). The wavelengths for the detection of arbutin, hydroquinone, niacinamide, adenosine, ascorbyl glucoside and ethyl ascorbyl ether were 283, 289, 261, 257, 238 and 245 nm, respectively. Results This method exhibited good linearity ( R 2 ≥ 0.999), precision (expressed as relative standard deviation ( RSD ) < 2%) and mean recoveries (89.42–104.89%). The results obtained by monitoring 100 market samples showed that the detected levels of the tested materials are within the acceptable authorized concentration. Conclusion The method developed herein is simple and can be used for market survey and quality control of functional cosmetics.