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Determination of cholesterol oxides by gas chromatography‐flame ionization detection/mass selective detection and their occurence in lanolin‐containing cosmetics and ointments
Author(s) -
Schrack S.,
Hohl C.,
Schwack W.,
Niederer M.,
Roux B.
Publication year - 2016
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/ics.12264
Subject(s) - lanolin , cosmetics , chemistry , chromatography , flame ionization detector , gas chromatography , organic chemistry
Synopsis Objective Cholesterol oxides ( COP s) are thought to be of toxicological relevance in cholesterol‐containing foods. For cholesterol‐containing cosmetics and the like, no information is available up to this date. Therefore, the first of two main aims of this study was to develop and validate a method for determining COP s in lanolin‐containing cosmetics such as lipsticks and fatty creams as well as in nipple ointments. The second aim was to study the occurrence of COP s and their concentration levels in the respective product classes. Methods The procedure is based on a published method for food comprising some necessary modifications. Sample preparation consisted of transesterfication, solid‐phase extraction and silylation of target compounds. Separation of the derivatized COP s and their quantification were performed with gas chromatography ( GC ) using a flame ionization detector ( FID ) or a mass spectrometer ( MS ). Results The successful validation and the trouble‐free application during the market survey showed that the method was fit for purpose. Total COP levels found were in the low per cent range (up to 3%) and surprisingly high, being many orders of magnitude higher than those published for foods. Conclusion To our knowledge, we present for the first time a method for the determination of COP s in non‐food consumer products. Furthermore, our study demonstrates that lanolin‐containing cosmetics may be an additional exogenous source of COP s. We further show evidence, that at least part of the COP s are already formed on the sheep's wool.