Premium
PDGF‐AA‐induced filamentous mitochondria benefit dermal papilla cells in cellular migration
Author(s) -
Mifude C.,
Kaseda K.
Publication year - 2015
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/ics.12190
Subject(s) - microbiology and biotechnology , platelet derived growth factor receptor , mitochondrion , biology , growth factor , dermal papillae , chemistry , biochemistry , hair follicle , receptor
Synopsis Objectives Human dermal papilla cells ( HDPC s) play essential roles in hair follicular morphogenesis and postnatal hair growth cycles. Previous reports demonstrated that platelet‐derived growth factor‐ AA ( PDGF ‐ AA ) enhanced the formation of dermal condensates in hair follicular development. Additionally, PDGF ‐ AA induces/maintains the anagen phase of the hair cycle. It is likely that mitochondrial morphology and functions are tightly coupled with maintenance of these energy‐demanding activities. However, little is known about the mitochondrial regulation in HDPC s. Thus, we investigated the PDGF ‐involved mitochondrial regulation in HDPC s. Methods The mitochondrial morphologies of HDPC s were examined in the presence or absence of PDGF ‐ AA under a fluorescent microscope. ATP production and cellular motility were investigated. The relationship between mitochondrial morphology and the cellular functions was discussed. Results We observed that primary HDPC s contained mitochondria with filamentous and/or rounded morphologies. Both types of mitochondria showed similar membrane potentials. Interestingly, in the presence of PDGF ‐ AA , but not PDGF ‐ BB , the balance between the two morphologies shifted towards the filamentous form. Concomitantly, both mitochondrial enzymatic activity and total cellular ATP level were augmented by PDGF ‐ AA . These two parameters were closely correlated, suggesting the mitochondrial involvement in the PDGF ‐augmented ATP production. Moreover, PDGF ‐ AA accelerated the migration of HDPC s in a gap‐filling assay, but did not change the rate of cellular proliferation. Notably, filamentous mitochondria dominated migrating HDPC s. Conclusion PDGF ‐ AA benefits HDPC s in the process of migration, by increasing the number of filamentous mitochondria.