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Characterization of a human epidermis model reconstructed from hair follicle keratinocytes and comparison with two commercially models and native skin
Author(s) -
Guiraud B.,
HernandezPigeon H.,
Ceruti I.,
Mas S.,
Palvadeau Y.,
SaintMartory C.,
CastexRizzi N.,
Duplan H.,
BessouTouya S.
Publication year - 2014
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/ics.12150
Subject(s) - involucrin , loricrin , filaggrin , epidermis (zoology) , stratum spinosum , stratum granulosum , stratum corneum , outer root sheath , keratinocyte , human skin , hair follicle , corneocyte , biology , microbiology and biotechnology , anatomy , cell culture , immunology , genetics , atopic dermatitis
Synopsis Objective Outer root sheath ( ORS ) cells of human hair follicles are a readily available, non‐invasive source of keratinocytes for epidermis reconstruction. The aim of this study was to characterize a model of epidermis reconstructed from ORS cells ( ORS ‐derived model) and to evaluate its reproducibility, in comparison with native human skin and two marketed reconstructed skin models (model A, Episkin ® and model B, Skinethic ® ). Methods Cell morphology and tissue architecture of the three models were analysed histologically and proliferation and differentiation marker expression by immunohistochemistry and m RNA quantification. Results All models displayed the same general epidermal architecture as native epidermis, but with a thicker stratum corneum in models A and B. Compared with native epidermis, Ki67 was correctly localized in epidermal basal cells in all models, as K10 in suprabasal layers. In all skin models, transglutaminase 1 ( TGM 1) was prematurely expressed in suprabasal layers. However, this expression was only observed from the upper stratum spinosum in the ORS ‐derived model. In this model, filaggrin and loricrin were correctly located in the stratum granulosum . Filaggrin, involucrin, loricrin and TGM 1 m RNA s (markers of keratinocyte terminal differentiation) were transcriptionally expressed in all models. In the ORS ‐derived model, transcriptional expression level was similar to that of native skin. Conclusion ORS cell‐based reconstructed epidermis is a valid and reproducible model for human epidermis and it may be used to evaluate the effects of active substances and cosmetic formulations.