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An efficient and rapid thin‐layer chromatography method for the identification of 32 dye substances in hair dye products
Author(s) -
Zhu H. J.,
Yang Y. W.,
Zhu Y.
Publication year - 2014
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/ics.12135
Subject(s) - hair dyes , thin layer chromatography , chromatography , reproducibility , chemistry , thin layer , acid dye , layer (electronics) , organic chemistry , dyeing
Synopsis Objective The use of dye substances in hair dye products has led to controversial public and scientific discussions about their impact on human health. This study aimed to explore a rapid method for identification of dye substances in hair dye products. Methods Thin layer chromatography (TLC) method was conducted in this study. We developed basic data of 32 dye substances by 4 developing solvents and 2 indicator sprays. The dye substances were identified by comparing the R f values and colors between samples and reference substances. Results Thirty samples were identified by the established method. 22 hair dye substances were detected in 16 formula known samples, 15 hair dye substances were detected in 14 formula unknown samples. Four laboratories participated in the validation and the results showed good interlaboratory reproducibility. Conclusion The developed TLC method is simple, rapid, reliable and can be finely used for identification of dye substances in hair dye products.