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In vitro anti‐ Malassezia activity and potential use in anti‐dandruff formulation of Asparagus racemosus
Author(s) -
Onlom C.,
Khanthawong S.,
Waranuch N.,
Ingkaninan K.
Publication year - 2014
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/ics.12098
Subject(s) - asparagus racemosus , dandruff , saponin , traditional medicine , broth microdilution , chemistry , malassezia , minimum inhibitory concentration , asparagus , food science , shampoo , antimicrobial , biology , botany , microbiology and biotechnology , medicinal plants , medicine , alternative medicine , organic chemistry , pathology
Synopsis Objective Malassezia species are frequently associated with dandruff and seborrhoeic dermatitis. The study was conducted to evaluate anti‐fungal activities of the extracts obtained from the roots of Asparagus racemosus Willd against Malassezia furfur and M. globosa . Methods Asparagus racemosus roots were successively extracted with the series of solvents, that is, hexane, ethanol and water, and also a saponin‐enriched fraction was prepared. The amounts of saponin (equivalent to shatavarin IV) in the extracts were determined using ELISA. The extracts were tested for anti‐fungal activity by disc diffusion and broth microdilution methods. Results By disc diffusion, only the ethanolic and saponin‐enriched extracts demonstrated anti‐fungal activity against M. furfur and M. globosa at the concentration of 1 mg per disc whereas the extracts with other solvents were ineffective. Multiple concentrations using the broth microdilution method against M. furfur and M. globosa yielded minimum inhibitory concentrations (MICs) of 25 mg mL −1 for the ethanolic extract but much higher potency for the saponin‐enriched extract: MICs to 0.20 and 0.40 mg mL −1 for M. furfur and M. globosa , respectively. These extracts showed no antagonist effect with the anti‐fungal agents, ketoconazole and zinc pyrithione. Conclusion These studies revealed the antifungal activity of A. racemosus roots extracts. Because A. racemosus is also anti‐inflammatory agent, it has the potential use as an active ingredient in an anti‐dandruff formulation.