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Whitening and antioxidant activities of bornyl acetate and nezukol fractionated from C ryptomeria japonica essential oil
Author(s) -
Kim S. H.,
Lee S. Y.,
Hong C. Y.,
Gwak K. S.,
Park M. J.,
Smith D.,
Choi I. G.
Publication year - 2013
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/ics.12069
Subject(s) - dpph , chemistry , ethyl acetate , cryptomeria , japonica , tyrosinase , antioxidant , food science , organic chemistry , chromatography , botany , biology , enzyme
Synopsis Objectives The aim of this study was to investigate the whitening and antioxidant activities of essential oils from Cryptomeria japonica by determining their tyrosinase inhibition, 1,1‐diphenyl‐2‐picrylhydrazyl ( DPPH ) radical scavenging and superoxide dismutase ( SOD )‐like activities. Methods Essential oils of C. japonica leaves were extracted with distilled water, and after condensation of volatile constituents, the condensates were extracted with ethyl acetate. Crude essential oils of C. japonica were divided into six fractions by thin layer chromatography and open column chromatography, and their chemical analysis was performed by GC / MS . Major compounds of fractions were composed of kaurene, bornyl acetate, nezukol, (‐)‐4‐terpineol, δ‐cadinene, α‐terpineol, γ‐eudesmol, α‐eudesmol and elemol. Results For tyrosinase inhibitory activity using two substrates, l ‐tyrosine and 3,4‐dihydroxyphenylalanine ( l ‐ DOPA ), kaurene, bornyl acetate and nezukol were highly effective. In antioxidant activity, (‐)‐4‐terpinenol and δ‐cadinene showed high DPPH radical scavenging activity, and bornyl acetate and nezukol indicated extremely high SOD ‐like activity. Conclusion Therefore, bornyl acetate and nezukol fractionated from C. japonica essential oil, which showed highly active whitening and antioxidant activities, have potential applications in cosmeceutical materials.