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N ‐Acetylglucosamine modulates function of the skin fibroblasts
Author(s) -
Połubinska A.,
Cwalinski J.,
Baum E.,
Bręborowicz A.
Publication year - 2013
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/ics.12067
Subject(s) - fibroblast , in vitro , ageing , chemistry , population , interleukin 8 , microbiology and biotechnology , immunology , andrology , biology , medicine , biochemistry , inflammation , environmental health
Synopsis Background Fibroblasts are an important component of the skin determining its properties. N ‐Acetylglucosamine ( NAG ) is the substrate for hyaluronan synthesis, and it also has anti‐inflammatory and anti‐senescent activity in mesothelial cells. Methods We tested in in vitro ‐cultured human skin fibroblasts how supplementation of culture medium with NAG 10 mmol L −1 changes properties of these cells. Results Fibroblasts cultured in presence of NAG produced more proteins and that was mainly due to increased synthesis of collagen (+33% vs. control, P < 0.05). Hyaluronan synthesis was increased (+107% vs. control, P < 0.001), but interleukin‐6 synthesis was reduced (−22% vs. control, P < 0.05). Fibroblasts cultured in medium with NAG 10 mmol L −1 demonstrated improved ability to heal the injured layer of cells (+34% vs. control, P < 0.05). Additionally senescence of fibroblasts undergoing replicative ageing in the presence of NAG was less pronounced, as reflected by smaller increase in the population doubling time (−70% vs. control, P < 0.05). Conclusion We conclude that NAG induced changes in the skin fibroblasts' properties maybe important for prevention of the age‐dependent changes in its structure and function.