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Determination of seven preservatives in cosmetic products by micellar electrokinetic chromatography
Author(s) -
Huang JunQiang,
Hu ChoChun,
Chiu TaiChia
Publication year - 2013
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/ics.12040
Subject(s) - chemistry , chromatography , micellar electrokinetic chromatography , preservative , paraben , bromide , detection limit , resorcinol , phenol , pulmonary surfactant , organic chemistry , biochemistry
Synopsis A micellar electrokinetic chromatography method using cetyltrimethylammonium bromide (CTAB) as a cationic surfactant, coupled with UV–Vis detection, was developed for the simultaneous determination of seven preservatives, including methyl‐, ethyl‐, propyl‐ and butyl‐paraben and phenol, phenoxyethanol and resorcinol. The method involved optimizing the pH of the phosphate buffer and concentrations of CTAB, ethanol and 2‐hydroxypropyl‐β‐cyclodextrin (HP‐β‐CD). The preservatives were well separated using optimum conditions and separated within 10 min at a separation voltage of −12.5 kV with the 1.0 mM phosphate buffer ( pH 7.0) containing 90 mM CTAB, 25 mM HP‐β‐CD and 10% (v/v) ethanol. Satisfactory recoveries (84.1–103.0%), migration time (RSD < 3.1%) and peak area (RSD < 4.5%) repeatabilities were achieved. Detection limits of the preservatives were between 0.31 and 1.52 μg mL −1 (S/N = 3, n = 5). The optimized method was successfully applied to the simultaneous determination of these preservatives in 10 commercial cosmetic products.