Open AccessIdentification of fruit related microRNAs in cucumber ( Cucumis sativus L.) using high‐throughput sequencing technology
Author(s) -
Ye Xueling,
Song Tiefeng,
Liu Chang,
Feng Hui,
Liu Zhiyong
Publication year - 2014
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/hrd2.00057
Subject(s) - cucumis , biology , microrna , deep sequencing , small rna , gene , dna sequencing , genetics , rna , computational biology , botany , genome
MicroRNAs (miRNAs) are approximately 21 nt noncoding RNAs that influence the phenotypes of different species through the post‐transcriptional regulation of gene expression. Although many miRNAs have been identified in a few model plants, less is known about miRNAs specific to cucumber ( Cucumis sativus L.). In this study, two libraries of cucumber RNA, one based on fruit samples and another based on mixed samples from leaves, stems, and roots, were prepared for deep‐sequencing. A total of 110 sequences were matched to known miRNAs in 47 families, while 56 sequences in 46 families are newly identified in cucumber. Of these, 77 known and 44 new miRNAs were differentially expressed, with a fold‐change of at least 2 and p‐value < 0.05. In addition, we predicted the potential targets of known and new miRNAs. The identification and characterization of known and new miRNAs will enable us to better understand the role of these miRNAs in the formation of cucumber fruit.