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HPV load and anal cytological abnormalities
Author(s) -
Maia LB,
Marinho LC,
Carneiro MV,
Bocca AL,
Neto FFC,
Motoyama AB,
Muniz Junqueira MI,
Ferreira VMM,
Carneiro FP,
Oliveira PG
Publication year - 2016
Publication title -
hiv medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.53
H-Index - 79
eISSN - 1468-1293
pISSN - 1464-2662
DOI - 10.1111/hiv.12317
Subject(s) - medicine , cytology , anal cancer , viral load , biopsy , gastroenterology , pathology , human papillomavirus , human immunodeficiency virus (hiv) , virology
Objectives The objective of this study was to evaluate the association between estimated human papillomavirus ( HPV ) viral load and abnormal cytology on anal samples. Methods Anal cytological samples of 42 HIV ‐positive patients were analysed by conventional cytology and Hybrid Capture II . Results On cytology, 30.95% (13 of 42) anal samples were positive for cytological abnormalities, 47.61% (20 of 42) were negative and 21.42% (nine of 42) were unsatisfactory. High‐risk HPV infection was more frequent in anal samples with cytological abnormalities than in negative samples ( P = 0.0002, Fisher's exact test), it was detected in all samples with cytological abnormalities and in 35% (seven of 20) of the negative samples. On samples with cytological abnormalities, the median of the relative light unit/cutoff ( RLU / CO ) value (viral load estimate) was 10.39 (1.02–572.6) and in negative samples it was 0.51 (0.26–51.70). The median of the RLU / CO value was higher in samples with cytological abnormalities when compared with the median in negative samples ( P = 0.0001, Mann–Whitney U ‐test) and only samples with cytological abnormalities showed RLU / CO values > 100. Conclusions The estimated high‐risk HPV viral load is significantly higher in samples with cytological abnormalities than in negative anal samples and may be useful as an adjunct to anal cytology for triage of patients to high‐resolution anoscopy and biopsy.