Premium
Liquid‐based cytological and immunohistochemical study of nasopharyngeal swab from persons under investigation for SARS‐CoV‐2 infection
Author(s) -
Parada David,
Peña Karla B,
Gumà Josep,
Guilarte Carmen,
Riu Francesc
Publication year - 2021
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/his.14257
Subject(s) - immunohistochemistry , virus , virology , pathology , nucleoprotein , cytology , antibody , medicine , covid-19 , monoclonal antibody , biology , immunology , disease , infectious disease (medical specialty)
We describe cytologic and immunohistologic findings in virus transport medium on cases under investigation of SARS‐CoV‐2 infection. Methods Cytologic findings in cases under investigation of SARS‐CoV‐2 infection from one hundred consecutive nasopharyngeal swab were reviewed. Immunohistochemistry and SARSCoV‐2 RT‐PCR determination were performed to detect virus. Results No viral inclusions were noted in squamous cells obtained from virus transport medium. Immunohistochemical study with monoclonal antibody against SARS‐CoV‐2 viral nucleoprotein showed positivity in squamous cells. No positivity was present in others cellular components. Conclusions SARS‐CoV‐2 predominantly localizes squamous cells in cytology samples of patients with RT‐PCR positive determination of SARSCoV‐2. The results of the current study support the notion that the nasopharyngeal region is the anatomical station that SARS‐CoV‐2 infects first, and the infection can lead to the migration of the virus into the lower airways.