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Differential clinical impacts of tumour budding evaluated by the use of immunohistochemical and haematoxylin and eosin staining in stage II colorectal cancer
Author(s) -
Yamadera Masato,
Shinto Eiji,
Kajiwara Yoshiki,
Mochizuki Satsuki,
Okamoto Koichi,
Shimazaki Hideyuki,
Hase Kazuo,
Ueno Hideki
Publication year - 2019
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/his.13830
Subject(s) - haematoxylin , immunohistochemistry , eosin , staining , stage (stratigraphy) , colorectal cancer , h&e stain , pathology , tumor budding , medicine , biology , cancer , metastasis , paleontology , lymph node metastasis
Abstract Aims The aim of this study was to clarify the quantitative and qualitative differences in tumour budding identification between haematoxylin and eosin (H&E) staining and immunohistochemical ( IHC ) staining for cytokeratin, and to estimate the respective clinical impacts in stage II colorectal cancer. Methods and results We retrospectively examined 314 surgically resected cases of stage II colorectal cancer, and assessed tumour budding on serial section slides with H&E staining and IHC staining for cytokeratin. Tumour budding counts based on cytokeratin‐stained slides were strongly correlated with those based on H&E‐stained slides, and had higher detection and reproducibility. On the basis of receiver operating characteristic analyses, the optimal cut‐off values of budding counts for relapse‐free survival ( RFS ) were 7 and 16 in a ×200 microscopic field with H&E and IHC staining, respectively. With these cut‐off values, tumour budding based on H&E staining had a significant correlation with RFS (80.3% and 93.2% of 5‐year RFS in the high‐budding group and the low‐budding group, respectively), and similar results were observed for IHC staining (79.9% and 91.7%, respectively). The Akaike Information Criterion value for RFS with H&E staining was favourable as compared with that with IHC staining. Conclusions Tumour budding counts based on cytokeratin‐stained slides showed higher detection and better reproducibility, but did not have as satisfactory clinical impacts as those based on H&E staining.