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Amplification of FRS2 in atypical lipomatous tumour/well‐differentiated liposarcoma and de‐differentiated liposarcoma: a clinicopathological and genetic study of 146 cases
Author(s) -
Jing Wenyi,
Lan Ting,
Chen Huijiao,
Zhang Zhang,
Chen Min,
Peng Ran,
He Xin,
Zhang Hongying
Publication year - 2018
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/his.13473
Subject(s) - liposarcoma , biology , medicine , pathology , sarcoma
Aims The aim of this study was to evaluate the frequency of FRS2 amplification and its relationship with the clinicopathological features of atypical lipomatous tumour (ALT)/well‐differentiated liposarcoma (WDL)/de‐differentiated liposarcoma (DDL). Methods and results FRS2 and MDM2 fluorescence in‐situ hybridisation (FISH) was performed on 146 tumours (70 ALT/WDLs and 76 DDLs). One hundred and eight control samples were included for FRS2 analysis. FRS2 amplification was detected in 136 of 146 (93.2%) ALT/WDL/DDLs, including 63 ALT/WDLs and 73 DDLs. A higher FRS2 /CEP12 ratio was observed in DDLs than in ALT/WDLs ( P = 0.0005). The FRS2 /CEP12 ratio of peripheral tumours was lower than that of central tumours ( P = 0.00004). All the ALT/WDL/DDLs showed MDM2 amplification (100%). The MDM2 + /FRS2 − series included seven ALT/WDLs and three DDLs. Four of seven (57.1%) MDM2 + /FRS2 − ALT/WDLs occurred in peripheral sites, slightly higher than the percentage of MDM2 + / FRS2 + ALT/WDLs (28 of 63, 44.4%). All the three MDM2 + /FRS2 − DDLs (100%) were peripheral tumours, a much higher proportion than that of MDM2 + / FRS2 + DDLs (10 of 73, 13.7%). A high percentage of homologous pleomorphic liposarcoma‐like DDLs (two of three) were observed in the MDM2 + / FRS2 − group. In the control group all the parosteal osteosarcomas (five of five, 100%) were FRS2 amplified, whereas the remaining 103 samples were FRS2 non‐amplified. Conclusions These findings suggest that FRS2 is amplified consistently in ALT/WDL/DDLs and offer another avenue for the investigation of the biology of this tumour group. MDM2 + / FRS2 − cases seem to be associated with certain clinicopathological features, and further investigation is needed.