Premium
Role of CD15 expression in dysplastic and neoplastic tissue of the bile duct – a potential novel tool for differential diagnosis of indeterminate biliary stricture
Author(s) -
Walter Dirk,
Herrmann Eva,
Winkelmann Ria,
Albert Jörg G,
Liese Juliane,
Schnitzbauer Andreas,
Zeuzem Stefan,
Hansmann MartinLeo,
PevelingOberhag Jan,
Hartmann Sylvia
Publication year - 2016
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/his.13041
Subject(s) - cd15 , dysplasia , bile duct , medicine , pathology , differential diagnosis , gastroenterology , biology , genetics , stem cell , cd34
Background and aims CD 15 is expressed by various cancer types; among these are intrahepatic and perihilar cholangiocarcinoma ( CCA ). The aim of this study was to elucidate CD 15 expression in distal CCA as well as in dysplastic biliary tissue and to determine its prognostic significance. Methods and results Tissue samples from patients with intrahepatic ( iCCA , n = 22), perihilar ( pCCA , n = 7) and distal CCA ( dCCA , n = 15), who underwent surgical resection in the period from 2010 to 2015 were evaluated for CD 15 expression. Tissue of synchronous lymph node metastasis ( n = 13), CCA ‐associated dysplasia ( n = 20), dysplasia in intraductal biopsies ( n = 10) and benign proliferations ( n = 12), as well as inflammatory biliary lesions ( n = 28) and non‐inflammatory bile ducts ( n = 23), were evaluated equally for CD 15 expression. CD 15 was found to be expressed highly in iCCA (81.8%), pCCA (85.7%), dCCA (73.3%), CCA ‐associated dysplasia (70.0%), dysplasia in intraductal biopsies (100%) and metastatic tissue (84.6%). CD 15 expression was negative in 58 of 64 benign bile duct alterations resulting in an overall sensitivity and specificity of CD 15 in CCA of 80.7 and 90.6% patients, respectively. CD 15 expression was correlated significantly with a decreased overall survival in patients with CD 15‐positive CCA associated dysplasia ( P = 0.003). However, CD 15 expression in the invasive tumour component was not correlated with clinical outcome. Conclusion CD 15 is a sensitive and specific marker for intraepithelial and invasive neoplasias of the bile duct. Therefore, it can be helpful in the delineation of dysplastic and neoplastic biliary cells from non‐neoplastic tissue, which frequently causes a diagnostic problem in indeterminate biliary stricture.