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Binding of the monoclonal antibody RP 215 to immunoglobulin G in metastatic lung adenocarcinomas is correlated with poor prognosis
Author(s) -
Liu Yang,
Liu Dan,
Wang Chong,
Liao Qinyuan,
Huang Jing,
Jiang Dongyang,
Shao Wenwei,
Yin Cheng Cameron,
Zhang Youhui,
Lee Gregory,
Qiu Xiaoyan
Publication year - 2015
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/his.12686
Subject(s) - immunostaining , lung cancer , immunohistochemistry , adenocarcinoma , flow cytometry , metastasis , pathology , gene knockdown , monoclonal antibody , antibody , medicine , cancer research , cancer , biology , immunology , cell culture , genetics
Aims Cancer cell‐derived immunoglobulin (Ig)G (cancer‐IgG) has been found to be involved in the pathogenesis and progression of many cancers, including lung cancer. The aim of the present study was to investigate the relationship between cancer‐IgG expression in lung adenocarcinoma ( ADC ) and clinicopathological characteristics and clinical outcome. Methods and results Immunohistochemical analysis was performed using an RP 215 monoclonal antibody to determine cancer‐IgG expression in 140 lung ADC patients. Cell migration and invasion were analysed in A549 cell line after short interfering RNA (si RNA ) knockdown of IgG and cell sorting by flow cytometry. Our results show that RP 215 immunostaining score is correlated significantly with local invasion ( P < 0.05) and tumour differentiation ( P < 0.05) in ADC . Moreover, RP 215 staining was significantly higher in metastatic tumours than in primary tumours ( P < 0.0001). The knockdown of IgG resulted in a reduction of cell migration and invasion. In contrast, RP 215‐positive cells displayed greater migration and invasion ability than RP 215‐negative cells. Additionally, a higher RP 215 immunostaining score was associated significantly with poor prognosis. Conclusions RP 215 staining is correlated strongly with differentiation, local invasion, metastasis and clinical outcome of patients with lung ADC . Our results suggest that RP 215 can serve as a biomarker for prognosis of lung ADC .