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The prognostic role of the epithelial–mesenchymal transition markers E‐cadherin and Slug in laryngeal squamous cell carcinoma
Author(s) -
Cappellesso Rocco,
Marioni Gino,
Crescenzi Marika,
Giacomelli Luciano,
Guzzardo Vincenza,
Mussato Alessio,
Staffieri Alberto,
Martini Alessandro,
Blandamura Stella,
Fassina Ambrogio
Publication year - 2015
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/his.12668
Subject(s) - slug , cdh1 , epithelial–mesenchymal transition , cadherin , immunohistochemistry , metastasis , cancer research , oncology , medicine , carcinoma , pathology , cancer , cell , biology , genetics
Aims Laryngeal squamous cell carcinoma ( LSCC ) prognosis is definitely related to lymph node metastasis. Epithelial–mesenchymal transition ( EMT ) allows neoplastic cells to gain the plasticity and motility required for tumour progression and metastasis. The aim of this study was to investigate the role of EMT in the prognosis of LSCC . Methods and results Immunohistochemical analysis of E‐cadherin, N‐cadherin, Snail, Slug, ZEB 1, and ZEB 2 was performed in 37 consecutive LSCC cases. Low E‐cadherin levels and high Slug levels correlated with both disease recurrence ( P = 0.02 and P =0.01, respectively) and shorter disease‐free survival ( DFS ) ( P = 0.04 and P = 0.02, respectively). Relative expression levels of CDH 1 , SNAI 2 , miR‐1 and the miR‐200 family were also evaluated. CDH 1 , miR‐200a and miR‐200c down‐regulation and SNAI 2 overexpression were significantly associated with disease recurrence ( P = 0.03, P = 0.02, P = 0.04, and P = 0.04, respectively). Conclusions EMT increases tumour recurrence risk and shortens DFS in LSCC . E‐cadherin and Slug immunohistochemical analysis could be useful for identifying patients requiring more aggressive treatment after surgery.