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Carnoy's solution is an adequate tissue fixative for routine surgical pathology, preserving cell morphology and molecular integrity
Author(s) -
Pereira Marina A,
Dias Andre R,
Faraj Sheila F,
Cirqueira Cinthya dos S,
Tomitao Michele T,
Carlos Nahas Sergio,
Ribeiro Ulysses,
Mello Evandro S
Publication year - 2015
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/his.12532
Subject(s) - fixative , sanger sequencing , fixation (population genetics) , surgical pathology , immunohistochemistry , pathology , colorectal cancer , polymerase chain reaction , biology , medicine , cancer , staining , dna , dna sequencing , biochemistry , gene
Aims To compare Carnoy's solution ( CS ) and 10% neutral buffered formalin solution ( NBF ) as tissue fixatives in colorectal cancer specimens. Methods and results Surgical specimens from patients with colorectal cancer were analysed. Three groups were studied, as follows: group 1 consisted of 16 paired samples fixed in CS and NBF ; and groups 2 and 3 consisted of 14 prospective and 80 retrospective samples, respectively, both randomized for fixation in CS or NBF . Groups 1 and 2 were analysed for amount, quality and integrity of DNA . Morphological analysis, including some of the usual special stains and polymerase chain reaction ( PCR ), were also performed for group 1, and Sanger sequencing for group 2. Immunohistochemical ( IHC ) reactions for mismatch repair proteins were studied in groups 1 and 3. Fixative performances were similar for morphology, special stains, and IHC reactions, as well as for the amount, quality and integrity of extracted DNA . PCR amplification was not possible in two cases from CS group 1. Sanger sequencing gave conclusive results for the CS samples tested. Conclusions Carnoy's solution and NBF are equivalent fixatives for colorectal cancer specimens and are adequate for routine utilization in surgical and molecular pathology.