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Decreased expression of P54 nrb /NonO correlates with collagen deposition and fibrosis in human aortic dissection
Author(s) -
Ren Zongli,
Wang Zhiwei,
Hu Zhipeng,
Hu Xiaoping,
Zhang Hao,
Wu Hongbing,
Hu Rui,
Liu Huan
Publication year - 2014
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/his.12434
Subject(s) - fibrosis , extracellular matrix , western blot , pathology , immunohistochemistry , chemistry , microbiology and biotechnology , biology , medicine , biochemistry , gene
Aims Aortic dissection ( AD ) is characterized by changes in the extracellular matrix, including fibrosis with collagen production. P54 nrb /NonO is known to be involved in collagen formation. In this study, we examined whether AD is associated with abnormal P54 nrb /NonO expression. Methods and results Aortic specimens and serum were obtained from 10 patients with AD and 10 controls. In‐vitro cultures of vascular smooth muscle cells (VSMCs) and adventitial fibroblasts (AFs) were obtained from organ donors. P54 nrb /NonO protein and mRNA levels were determined by Western blot, immunohistochemistry and quantitative real‐time reverse transcription–polymerase chain reaction (quantitative real‐time RT–PCR). To evaluate collagen expression, we stained tissue sections with Masson's trichrome. Serum concentration of TNF‐α was determined by enzyme‐linked immunosorbent assay (ELISA). Aortic P54 nrb /NonO protein and mRNA were decreased in AD patients, compared with controls. Decreased P54 nrb /NonO mRNA correlated significantly with increased collagen deposition and fibrosis in AD aortas. In VSMCs and AFs from normal human aortas, P54 nrb /NonO was expressed strongly and localized to the nucleus. Conclusions Patients with AD exhibited significantly decreased expression of P54 nrb /NonO. The significant correlation between P54 nrb /NonO and collagen may point to novel thinking about collagen metabolism research in AD aorta.