Premium
HMGA 2 is down‐regulated by micro RNA let‐7 and associated with epithelial–mesenchymal transition in oesophageal squamous cell carcinomas of K azakhs
Author(s) -
Liu Qing,
Liu Tao,
Zheng Shutao,
Gao Xiangpeng,
Lu Mang,
Sheyhidin Ilyar,
Lu Xiaomei
Publication year - 2014
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/his.12401
Subject(s) - hmga2 , epithelial–mesenchymal transition , transfection , kazakh , cancer research , epithelium , biology , cell , microrna , cell growth , immunohistochemistry , pathology , transition (genetics) , cell culture , medicine , gene , genetics , linguistics , philosophy
Aims To investigate the expression of let‐7 and its regulation of high‐mobility group A2 protein ( HMGA 2), and to verify the relationship between let‐7, HMGA 2 and the process of epithelial–mesenchymal transition ( EMT ), in oesophageal squamous cell carcinomas ( OSCC ) of K azakh patients. Methods and results Expression of let‐7 was significantly lower in E ca109 cells than in normal oesophageal squamous epithelium ( P = 2.4 × 10 −7 ). Increased accumulation of let‐7 after transfection of Eca109 cells with synthetic let‐7 inhibited cell proliferation. Let‐7 could repress expression of HMGA 2 after co‐transfection with let‐7 and HMGA 2 ( P = 0.002). Moreover, let‐7 expression was observed less frequently ( P = 2.0 × 10 −8 ), and HMGA 2 expression more frequently ( P = 1.0 × 10 −10 ), in OSCC than in normal adjacent tissues; and let‐7 expression was observed less frequently in OSCC from K azakh patients than in those from Han and Uygur patients ( P = 0.041). There was a reverse correlation between expression of let‐7 and HMGA 2 ( P = 0.018). Expression of Snail was statistically higher in K azakhs' OSCC ( P = 0.029), and was correlated with depth of invasion ( P = 0.021) and HMGA 2 expression ( P = 0.026). Conclusions Expression of let‐7 can suppress cell proliferation by acting directly on regulation of HMGA 2 in OSCC . High expression of Snail and its correlation with HMGA 2 expression and tumour invasion suggest that HMGA 2 may be involved in EMT in the OSCC of Kazakh patients.