Premium
Molecular pathological analysis of sarcomas using paraffin‐embedded tissue: current limitations and future possibilities
Author(s) -
Rijn Matt,
Guo Xiangqian,
Sweeney Robert T,
Beck Andrew H,
West Robert B
Publication year - 2014
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/his.12290
Subject(s) - immunohistochemistry , pathology , sarcoma , chromosomal translocation , biology , molecular diagnostics , gene , computational biology , medicine , bioinformatics , genetics
Summary Sarcomas of soft tissue and bone are rare neoplasms that can be separated into a large number of different diagnostic entities. Over the years, a number of diagnostic markers have been developed that aid pathologists in reaching the appropriate diagnoses. Many of these markers are sarcoma‐specific proteins that can be detected by immunohistochemistry in formalin‐fixed, paraffin‐embedded ( FFPE ) sections. In addition, a wide range of molecular studies have been developed that can detect gene mutations, gene amplifications or chromosomal translocations in FFPE material. Until recently, most sequencing‐based approaches relied on the availability of fresh frozen tissue. However, with the advent of next‐generation sequencing technologies, FFPE material is increasingly being used as a tool to identify novel immunohistochemistry markers, gene mutations, and chromosomal translocations, and to develop diagnostic tests.