Premium
The clinicopathological significance and relationship of G li1, MDM 2 and p53 expression in resectable pancreatic cancer
Author(s) -
Sheng Weiwei,
Dong Ming,
Zhou Jianping,
Li Xin,
Liu QingFeng,
Dong Qi,
Li Feng
Publication year - 2014
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/his.12273
Subject(s) - gene knockdown , immunohistochemistry , gli1 , cancer research , pancreatic cancer , mutant , mdm2 , rna interference , stage (stratigraphy) , clinical significance , small hairpin rna , biology , cancer , medicine , chemistry , cell culture , rna , gene , microbiology and biotechnology , hedgehog signaling pathway , signal transduction , genetics , biochemistry , paleontology
Aims To study the expression of Gli1, MDM 2 and p53 for clinical significance in pancreatic cancer ( PC ), and their functional relationship in regulating the biological behaviour of PC cells. Methods and results Immunohistochemistry showed that the expression of G li1, MDM 2 and p53 was much higher in 57 cases of PC than in paired normal pancreatic tissues, and was positively associated with tumour UICC stage and T stage ( P < 0.05). Patients with expression of G li1 only or coexpression of G li1 and MDM 2 had significantly worse overall survival than patients with negative expression ( P < 0.05). RNA interference showed that p53 knockdown increased the protein level of G li1 but decreased the level of MDM 2, and enhanced cell invasion and migration in wild‐type p53 C apan‐2 cells; whereas G li1 or MDM 2 knockdown did not change p53 expression, but decreased the protein level of MDM 2 or G li1, respectively, and inhibited cell invasion and migration in mutant p53 PANC ‐1 cells. Conclusions Overexpression of G li1, MDM 2 and mutant p53 contributes to the development and progression of PC , and plays an important role in predicting PC patients’ prognosis. Moreover, we report a positive association between G li1 and MDM 2 in PC cells, but their relationship with p53 is dependent on wild‐type or mutant p53 status.