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CD 30 expression by bone marrow mast cells from different diagnostic variants of systemic mastocytosis
Author(s) -
Morgado José M,
Perbellini Omar,
Johnson Ryan C,
Teodósio Cristina,
Matito Almudena,
ÁlvarezTwose Iván,
Bonadonna Patrizia,
Zamò Alberto,
JaraAcevedo Maria,
Mayado Andrea,
GarciaMontero Andrés,
Mollejo Manuela,
George Tracy I,
Zanotti Roberta,
Orfao Alberto,
Escribano Luis,
SánchezMuñoz Laura
Publication year - 2013
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/his.12221
Subject(s) - systemic mastocytosis , bone marrow , flow cytometry , mast cell , pathology , medicine , immunology
Aims CD 30 expression by bone marrow ( BM ) mast cells ( MC ) has been reported recently in systemic mastocytosis ( SM ) patients. The aim of this study was to investigate the potential diagnostic and prognostic value of CD 30 expression in SM as assessed by multiparameter flow cytometry. Methods and results A total of 163 consecutive BM samples corresponding to 142 SM patients and 21 non‐mastocytosis cases were studied. CD 30 was positive in most SM patients (80%), but in only one non‐mastocytosis case (4.8%). When combined with CD 25, CD 30 contributed to an improved accuracy over that of CD 25 alone (98% versus 93%) mainly because most (eight of nine) of the well‐differentiated SM ( WDSM ), who lacked CD 25, were CD 30 + . Similar levels of expression of CD 30 were observed among all different subgroups of SM except mast cell leukaemia; among indolent SM ( ISM ) patients, no significant association was observed between the levels of CD 30 expression and other clinical and biological features of the disease. Conclusions The increased expression of CD 30 associated with absence of CD 25 contributes to the diagnosis of WDSM and its distinction from other subtypes of SM . By contrast, CD 30 expression did not contribute either to prognostic stratification of ISM or to the differential diagnosis between ISM and aggressive SM cases.