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Immunohistochemical analysis of mismatch proteins in carcinogenesis of the lower lip
Author(s) -
Sarmento Dmitry José de Santana,
Almeida Wagner Leite,
Miguel Márcia Cristina da Costa,
Queiroz Lélia Maria Guedes,
Godoy Gustavo Pina,
Cruz Maria Carmen Fontoura Nogueira,
Silveira Éricka Janine Dantas
Publication year - 2013
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/his.12197
Subject(s) - immunohistochemistry , dysplasia , carcinogenesis , lesion , pathology , lower lip , medicine , basal cell , carcinoma , cancer , biology , surgery
Aim This study investigated the immunohistochemical expression of hMLH 1 and hMSH 2 proteins in lower lip squamous cell carcinoma ( SCC ) and actinic cheilitis ( AC ), to contribute to the understanding of the development of lower lip cancer. Methods and results Forty cases of lower lip AC and SCC were studied. Q uantitative immunohistochemical analysis was undertaken by counting 1000 cells (positive and negative) in each lesion. Statistical evaluation included Student's t ‐test and one‐way anova . For SCC and AC , the mean number of hMLH 1‐ and hMSH 2‐positive cells decreased with advanced stage of the lesion. The largest mean number of immunostained cells was observed in AC cases without dysplasia or with mild dysplasia ( hMLH 1: 721.23 ± 88.116; hMHS 2: 781.50 ± 156.93). Intermediate values were obtained for AC with moderate or severe dysplasia ( hMLH 1: 532.86 ± 197.72; hMHS 2: 611.14 ± 172.48). Lower lip SSCs presented the smallest number of positive cells ( hMLH 1: 255.03 ± 199.47; hMHS 2: 518.38 ± 265.68). A statistically significant difference was observed between groups ( P < 0.001). Conclusions The results support the hypothesis that changes in the immunoexpression of these mismatch proteins are related to the process of carcinogenesis of the lower lip.