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MALDI imaging on large‐scale tissue microarrays identifies molecular features associated with tumour phenotype in oesophageal cancer
Author(s) -
Quaas Alexander,
Bahar Ahmad Soliaman,
Loga Katharina,
Seddiqi Ahmad Shoaib,
Singer Julius Magnus,
Omidi Maryam,
Kraus Olga,
Kwiatkowski Marcel,
Trusch Maria,
Minner Sarah,
Burandt Eike,
Stahl Phillip,
Wilczak Waldemar,
Wurlitzer Marcus,
Simon Ronald,
Sauter Guido,
Marx Andreas,
Schlüter Hartmut
Publication year - 2013
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/his.12193
Subject(s) - tissue microarray , pathology , cancer , mass spectrometry imaging , stage (stratigraphy) , maldi imaging , lymph node , phenotype , metastasis , cancer research , cell , adenocarcinoma , biology , immunohistochemistry , medicine , chemistry , mass spectrometry , matrix assisted laser desorption/ionization , gene , paleontology , biochemistry , organic chemistry , chromatography , adsorption , desorption , genetics
Aims Matrix‐assisted laser desorption/ionisation mass spectrometry imaging ( MALDI ‐ MSI ) and tissue microarray ( TMA ) technologies were jointly utilized to search for molecular features associated with clinicopathological parameters in oesophageal cancer. Methods and results Two TMAs from formalin‐fixed tissue samples, including 300 adenocarcinomas and 177 squamous cell carcinomas with clinical follow‐up data, were analysed. MALDI‐MSI analysis revealed 72 distinct mass per charge ( m / z ) signals associated with tumour cells, 48 of which were found in squamous cell carcinomas only, and 12 of which were specific for adenocarcinomas. In adenocarcinomas, six signals were linked to early‐stage ( pT 1–T2) tumours (two signals) and the presence (one signal) or absence (three signals) of lymph node metastasis. In squamous cell carcinomas, 24 signals were strongly linked to different phenotypic features, including tumour stage (four signals), histological grade (four signals), and lymph node metastasis (three signals). Conclusions The high number of m / z signals that were found to be significantly linked to one or more phenotypic features of oesophageal cancer highlights the power of MALDI ‐ MSI in the analysis of high‐density TMA s. The data also emphasise substantial biological differences between adenocarcinomas and squamous cell carcinomas.