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Immunohistochemical analysis of the monoclonal antibody 4B5 in breast tissue expressing human epidermal growth factor receptor 4 (HER4)
Author(s) -
Jay Julie I,
Brunhoeber Patrick S,
Smith Margie H,
Williams Ryan R,
Sugarman Michael C,
Free Heather L,
Tast David E
Publication year - 2013
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/his.12024
Subject(s) - clone (java method) , immunohistochemistry , monoclonal antibody , antibody , epidermal growth factor receptor , breast carcinoma , biology , pathology , epidermal growth factor , microbiology and biotechnology , monoclonal , breast cancer , receptor , medicine , immunology , cancer , gene , biochemistry , genetics
Aims A recent study examining the specificity of human epidermal growth factor receptor ( HER ) 2 pharmacodiagnostic antibodies demonstrated that CB 11 and 4B5 stain both HER 2 ‐transfected and HER 4 ‐transfected cell lines. However, there has been no evidence showing that 4B5 has affinity for HER 4 in clinically obtained tissues, and, if so, whether this has any impact on the assessment of HER 2. We therefore sought to determine the expression of membrane‐bound HER 4 in clinical breast carcinomas, and evaluate its impact on the clinical utility of 4B5 in determining HER 2 status. Methods and results Breast carcinomas were assessed by immunohistochemistry ( IHC ) for membrane‐bound HER 4 using anti‐ HER 4 clone E200. HER 2 expression in these cases was then assessed using anti‐ HER 2 clone 4B5, and a reference clone, SP 3. In all 117 membrane HER 4‐positive cases (out of 241), 4B5 scored equal to or less than the reference anti‐ HER 2 clone SP 3. Eighteen cases were positive for membrane‐bound HER 4 by E200 and negative by 4B5, including a membrane HER 4 level 3+ case. Conclusions No cross‐reactivity of 4B5 with membrane‐bound HER 4 was identified in the clinical IHC analysis of formalin‐fixed paraffin‐embedded breast carcinoma cases as evidenced by the HER 4 antibody clone E200.