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Effects of a low‐intensity resistance exercise program on serum miR‐630, miR‐5703, and Fractalkine/CX3CL1 expressions in subjects with No exercise habits: A preliminary study
Author(s) -
Hashida Ryuki,
Matsuse Hiroo,
Kawaguchi Takumi,
Yoshio Sachiyo,
Bekki Masafumi,
Iwanaga Sohei,
Sugimoto Takahiro,
Hara Koji,
Koya Shunji,
Hirota Keisuke,
Nakano Dan,
Tsutsumi Tsubasa,
Kanto Tatsuya,
Torimura Takuji,
Shiba Naoto
Publication year - 2021
Publication title -
hepatology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.123
H-Index - 75
eISSN - 1872-034X
pISSN - 1386-6346
DOI - 10.1111/hepr.13670
Subject(s) - myokine , kegg , microrna , medicine , physical exercise , exercise intensity , endocrinology , gene expression , biology , gene , genetics , heart rate , transcriptome , skeletal muscle , blood pressure
Aims Exercise is effective for the prevention of liver cancer. Exercise exerts biological effects through the regulation of microRNAs (miRNAs) and cytokines/myokines. We aimed to investigate the effects of low‐intensity resistance exercise on serum miRNA and cytokine/myokine expressions in subjects with no exercise habits. Methods We enrolled seven male subjects with no exercise habits in this prospective before‐after study. All subjects performed a low‐intensity resistance exercise program (three metabolic equivalents, approximately 20 min/session). Serum miRNA expressions were evaluated using microarrays. We performed Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of differentially expressed miRNAs before and after exercise. Serum cytokine/myokine expressions were evaluated using a multiplex panel. Results All subjects completed the exercise program with no adverse events. In the microarray analysis, seven miRNAs showed a significant change between before and after exercise. Of these, microRNA (miR)‐630 and miR‐5703 showed a >1.5‐fold increase (miR‐630: 40.7 vs. 69.3 signal intensity, p = 0.0133; miR‐5703: 30.7 vs. 55.9 signal intensity, p = 0.0051). KEGG pathway enrichment analysis showed that miR‐630‐ and miR‐5703‐related genes were enriched in 37 and 5 pathways, including transforming growth factor‐beta and Wnt signaling pathways, respectively. In the multiplex analysis, 12 cytokines/myokines showed significant alteration after exercise compared to before exercise. Of these, fractalkine/CX3CL1 showed the most significant up‐regulation by exercise (94.5 vs. 109.1 pg/ml, p = 0.0017). Conclusions A low‐intensity resistance exercise program was associated with upregulation of serum miR‐630, miR‐5703, and fractalkine/CX3CL1 expressions in subjects with no exercise habits. Thus, even low‐intensity exercise may alter miRNA and cytokine/myokine expressions in humans.