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Photobiomodulation with red light‐emitting diodes accelerates hepatocyte proliferation through reactive oxygen species/extracellular signal‐regulated kinase pathway
Author(s) -
Feng Rui,
Morine Yuji,
Ikemoto Tetsuya,
Imura Satoru,
Iwahashi Shuichi,
Saito Yu,
Shimada Mitsuo
Publication year - 2018
Publication title -
hepatology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.123
H-Index - 75
eISSN - 1872-034X
pISSN - 1386-6346
DOI - 10.1111/hepr.13182
Subject(s) - reactive oxygen species , cell growth , hepatocyte , hepatocyte growth factor , microbiology and biotechnology , extracellular , intracellular , transplantation , cell cycle , cell , biology , cancer research , in vitro , biochemistry , medicine , receptor
Aim Cell‐based transplantation is an alternate method of liver transplantation to delay the onset of end‐stage liver diseases. For successful treatment, cells need to be expended in vitro expeditiously. However, autogenetic hepatocytes as the ideal cell source for therapy remain in quiescence so proliferation is rare. Photobiomodulation therapy has been used to stimulate some kinds of cell proliferation, but is unknown whether red light‐emitting diode (LED) irradiation can promote primary hepatocyte proliferation. The aim of this study was to evaluate the effect of red LED irradiation on hepatocytes in vitro . Methods Mouse primary hepatocytes were isolated and received red LED treatment. The cell viability, reactive oxygen species (ROS) levels, phosphorylated extracellular signal‐regulated kinase1/2 (pERK1/2) and some cell cycle‐related proteins were observed. Additionally, ROS inhibition and pERK1/2 inhibition were carried out to determine the effect of ROS and ERK1/2 in red LED irradiation. Results The red LED irradiation increased hepatocyte proliferation, elevated intracellular ROS levels, and stimulated ERK1/2 activation and cell cycle‐related gene expression. The mitosis promoting effect of red LED irradiation could be disturbed by ROS or pERK inhibition. The red LED irradiation promoted hepatocyte proliferation through the ROS/pERK1/2 pathway. Conclusions Red LED irradiation could accelerate hepatocyte proliferation through the ROS/pERK1/2 pathway. Red LED irradiation might be a potential method to increase hepatocyte cell numbers in vitro and support cell‐based transplantation in clinical work.