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lincRNA‐p21 inhibits invasion and metastasis of hepatocellular carcinoma through Notch signaling‐induced epithelial–mesenchymal transition
Author(s) -
Jia Meng,
Jiang Li,
Wang YaDong,
Huang JinZhao,
Yu Miao,
Xue HuanZhou
Publication year - 2016
Publication title -
hepatology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.123
H-Index - 75
eISSN - 1872-034X
pISSN - 1386-6346
DOI - 10.1111/hepr.12659
Subject(s) - downregulation and upregulation , epithelial–mesenchymal transition , metastasis , cancer research , notch signaling pathway , biology , carcinogenesis , transfection , cell , cell culture , signal transduction , microbiology and biotechnology , cancer , gene , genetics
Aim Emerging evidence has showed that long non‐coding RNA (lncRNA) play an important role in the occurrence and development of various cancers. In the present study, the expression level of lincRNA‐p21 was investigated in hepatocellular carcinoma (HCC), and its role in invasion of HCC was also explored. Methods The lincRNA‐p21 levels in human HCC tumor tissue and cell lines HepG2 and SMMC‐7721 were determined by real‐time polymerase chain reaction. Transfected HCC cells with pcDNA‐lincRNA‐p21 or si‐lincRNA‐p21 for overexpression or downregulation of lincRNA‐p21, the Notch signaling and epithelial–mesenchymal transition (EMT)‐related proteins and cell invasion were measured by western blot and Transwell assay, respectively. A tumor xenotransplant mouse model was also established to investigate the role of lincRNA‐p21 in tumor metastasis in vivo.Results The lincRNA‐p21 expression was downregulated in HCC tissue and cells. Overexpression of lincRNA‐p21 inhibited Notch singling and EMT, while its downregulation led to the reverse result. The invasion of HCC cell was also inhibited by pcDNA‐lincRNA‐p21, and activation of Notch signaling reversed this effect. In vivo , overexpression of lincRNA‐p21 decreased the tumor metastasis, as well. Conclusion lincRNA‐p21 was downregulated in HCC and lincRNA‐p21 overexpression contributed to the inhibition of tumor invasion through mediating Notch signaling induced EMT.