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An efficient system for secretory production of fibrinogen using a hepatocellular carcinoma cell line
Author(s) -
Matsumoto Michinori,
Matsuura Tomokazu,
Aoki Katsuhiko,
Maehashi Haruka,
Iwamoto Takeo,
Ohkawa Kiyoshi,
Yoshida Kiyotsugu,
Yanaga Katsuhiko,
Takada Koji
Publication year - 2015
Publication title -
hepatology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.123
H-Index - 75
eISSN - 1872-034X
pISSN - 1386-6346
DOI - 10.1111/hepr.12353
Subject(s) - fibrinogen , cell culture , cell , hepatocellular carcinoma , microbiology and biotechnology , secretion , biology , chemistry , andrology , immunology , biochemistry , endocrinology , cancer research , medicine , genetics
Aim Despite an increasing demand, blood products are not always safe because most are derived from blood donations. One possible solution is the development and commercialization of recombinant fibrinogen, but this process remains poorly developed. This study aimed to develop an effective production system for producing risk‐free fibrinogen using human hepatocellular cell lines and serum‐free media. Methods Three human liver cancer cell lines ( HepG2 , FLC ‐4 and FLC ‐7) were cultivated in a serum‐supplemented medium or two serum‐free media ( ASF104N and IS‐RPMI ) to compare their fibrinogen secretion abilities. Fibrinogen subunit gene expression was estimated by quantitative polymerase chain reaction. Massive fibrinogen production was induced using a 5‐mL radial flow bioreactor ( RFB ) while monitoring glucose metabolism. Subsequently, fibrinogen's biochemical characteristics derived from these cells were analyzed. Results FLC ‐7 cell culture combined with IS‐RPMI resulted in significantly better fibrinogen production (21.6 μg/10 7 cells per day). ASF104N had more positive effects on cell growth compared with IS‐RPMI , whereas fibrinogen production was more efficient with IS‐RPMI than with ASF104N . Changing the medium from ASF104N to IS‐RPMI led to significantly increased fibrinogen gene expression and glucose consumption. In the RFB culture, the fibrinogen secretion rate of FLC ‐7 cells reached 0.73 μg/mL per day during a 42‐day cultivation period. The subunit composition and clot formation activity of FLC ‐7 cell‐derived fibrinogen corresponded to those of plasma fibrinogen. Conclusion The FLC ‐7 cell culture system is suitable for large‐scale fibrinogen preparation production.