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Expression and clinical implications of HA b18 G / CD 147 in hepatocellular carcinoma
Author(s) -
Zhu Shaojun,
Li Yanhong,
Zhang Yang,
Wang Xuxia,
Gong Li,
Han Xiujuan,
Yao Li,
Lan Miao,
Zhang Wei
Publication year - 2015
Publication title -
hepatology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.123
H-Index - 75
eISSN - 1872-034X
pISSN - 1386-6346
DOI - 10.1111/hepr.12320
Subject(s) - hepatocellular carcinoma , cirrhosis , immunohistochemistry , medicine , metastasis , messenger rna , carcinoma , cancer research , biology , oncology , pathology , gene , cancer , biochemistry
Aim HA b18 G / CD 147 is an important factor in invasion and metastasis of hepatocellular carcinoma ( HCC ). However, the clinical implications of HA b18 G / CD 147 expression in HCC are still unclear. In this study, we clarify the clinical significance of HA b18 G / CD 147. We characterize the association between HA b18 G / CD 147 expression and presentation of fibrosis or chronic hepatitis B , as well as its effect on HCC development. Methods The expression of HA b18 G / CD 147 in human hepatocarcinoma cell lines was analyzed by reverse transcription polymerase chain reaction and western blotting. Tumor tissues were obtained from HCC patients who underwent surgical resection between 2002 and 2006. All patients who had received previous therapy were excluded. HCC tissues were analyzed by immunohistochemistry using anti‐ HA b18 G / CD 147. Results HA b18 G / CD 147 was widely expressed in H ep‐ G 2, SMCC ‐7721 and BEL 7402 cell lines, but not expressed in L ‐02, a human normal hepatic cell line. HA b18 G / CD 147 was mainly localized to the membrane of tumor cells in 74.0% (37/50) HCC patients. We found that higher HA b18 G / CD 147 expression and poor tumor differentiation were correlated with patient survival ( P = 0.026 and P = 0.014, respectively). Furthermore, the distribution of HA b18 G / CD 147 was similar to that of hepatitis B virus ( HBV ) infection, but negatively related to hepatic cirrhosis. Conclusion HA b18 G / CD 147 has shown its potentials in HCC development and patient survival. Moreover, it may also cooperate with chronic HBV infection and cirrhosis during HCC development. Its functions in the two factors may be different. Therefore, HA b18 G / CD 147 may be a marker for poor prognosis in HCC patients and could be a useful therapeutic target for interfering with or reversing HCC progression.