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Effect of HLA‐DP and IL28B gene polymorphisms on response to interferon treatment in hepatitis B e‐antigen seropositive chronic hepatitis B patients
Author(s) -
Cheng Lin,
Sun Xiaowen,
Tan Shun,
Tan Wenting,
Dan Yunjie,
Zhou Yi,
Mao Qing,
Deng Guohong
Publication year - 2014
Publication title -
hepatology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.123
H-Index - 75
eISSN - 1872-034X
pISSN - 1386-6346
DOI - 10.1111/hepr.12284
Subject(s) - hbeag , medicine , pegylated interferon , seroconversion , immunology , hepatitis b virus , genotype , single nucleotide polymorphism , virology , hepatitis b , interferon , human leukocyte antigen , gastroenterology , antigen , hbsag , hepatitis c virus , virus , biology , gene , ribavirin , biochemistry
Aim The reason why the majority of chronic hepatitis B ( CHB ) patients do not respond to conventional interferon ( IFN )‐α or pegylated interferon ( PEG IFN ) treatment has not been formally demonstrated. The aim of this study was to investigate the association between single nucleotide polymorphisms ( SNPs ) and response to IFN ‐α or PEG IFN therapy in C hinese patients with CHB . Methods Four SNPs among the HLA‐DPA1 , HLA‐DPB1 (rs3077 and rs9277535) and IL28B (rs12979860 and rs8099917) regions were genotyped using the MGB ‐ TaqMan SNP genotyping assay in 144 hepatitis B e‐antigen ( HB e A g) seropositive CHB patients who had received 6–12 months IFN ‐α or PEG IFN treatment. Patients were classified as responders who achieved any of the four targets: (i) the loss of HB e A g; (ii) anti‐ HBe seroconversion; (iii) suppression of hepatitis B virus ( HBV ) DNA level to below 3 log of baseline; and (iv) alanine aminotransferase normalization. Results By multivariate analysis at 6 months of therapy and 6 months post‐therapy, the results showed that rs3077‐ GG genotype was independently associated with higher HB e A g loss rate and anti‐ HBe seroconversion rate, and rs9277535‐ GG genotype was independently associated with decline of HBV DNA level. However, we did not observe the significant association between SNP near IL28B and the response to IFN ‐α or PEG IFN treatment. Conclusion This study suggested that HLA ‐ DPA1 and HLA ‐ DPB1 variants were significantly associated with HB e A g loss, anti‐ HBe seroconversion and HBV DNA level suppression in HB e A g seropositive CHB patients who received IFN ‐α or PEG IFN treatment.