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Splenic lymph follicles generate immunoglobulin M ‐producing B cells in primary biliary cirrhosis
Author(s) -
Kikuchi Kentaro,
Tsuneyama Koichi,
Yamada Hanae,
Kajiyama Yusuke,
Matsumoto Kotaro,
Tsunashima Hiromichi,
Yamashita Risa,
Takai Atsuko,
Negishi Masatsugu,
Hara Masumi,
Moritoki Yuki,
Miyakawa Hiroshi
Publication year - 2014
Publication title -
hepatology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.123
H-Index - 75
eISSN - 1872-034X
pISSN - 1386-6346
DOI - 10.1111/hepr.12231
Subject(s) - primary biliary cirrhosis , lymph , antibody , immunology , medicine , pathology
Aim To reveal the site of immunoglobulin ( Ig ) M production in primary biliary cirrhosis ( PBC ) we performed immunohistochemical analysis on spleens collected from patients with PBC . Methods Splenic tissue samples were collected at the time of the autopsy from patients with hepatic failure. Immunostaining for IgM , CD21 and CXCL13 were performed using the splenic tissue samples. Results The samples from five out of eight cases with PBC but not in eight cases of chronic hepatitis C virus infection showed accumulation of IgM positive cells in CD21 positive lymph follicles. The CXCL13 positive cells also accumulated in the center of the lymph follicles where the IgM positive cells accumulated. Conclusion The present results suggest that excess IgM is produced from the spleen of PBC . Furthermore, it was suggested that CXCL13 positive follicular dendritic cells possibly contribute to this process.