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Increased circulating L in −/low CD 33 + HLA‐DR − myeloid‐derived suppressor cells in hepatocellular carcinoma patients
Author(s) -
Shen Peng,
Wang Aijuan,
He Mengye,
Wang Qingqing,
Zheng Shusen
Publication year - 2014
Publication title -
hepatology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.123
H-Index - 75
eISSN - 1872-034X
pISSN - 1386-6346
DOI - 10.1111/hepr.12167
Subject(s) - hepatocellular carcinoma , peripheral blood mononuclear cell , myeloid derived suppressor cell , medicine , population , immunology , immune system , cancer research , cancer , tumor necrosis factor alpha , chemistry , suppressor , biochemistry , environmental health , in vitro
Aim Myeloid‐derived suppressor cells ( MDSC ) can be induced or expanded in tumor‐bearing mice and cancer patients. The frequency of MDSC denoted here as L in −/low CD 33 + HLA‐DR − was investigated in hepatocellular carcinoma ( HCC ) patients. The clinical relevance of MDSC and patients' characteristics were examined. Also, MDSC ‐related immune regulatory pathways in these patients were discussed. Methods The quantity of MDSC was tested in peripheral blood of patients with HCC ( n = 63) and healthy donors ( n = 56). The expressions of interferon ( IFN )‐γ, vascular endothelial growth factor ( VEGF ), cyclooxygenase ( COX )‐2, matrix metalloproteinase ( MMP )‐13, nitric oxide synthase ( NOS )‐2 and arginase ( ARG )‐1 were analyzed. Co‐culturing with anti‐ CD 3/ CD 28‐stimulated T lymphocytes was used to determine the suppressive effect of MDSC on the T lymphocytes.Results Patients with treatment‐naive HCC had an increased subpopulation of L in −/low CD 33 + HLA‐DR − cells in the peripheral blood mononuclear cells ( PBMC ) with characteristics of MDSC and associated to the stage ( P = 0.0004). Patients with splenomegaly had a higher frequency of circulating MDSC . Also, COX ‐2, MMP ‐13 and VEGF were expressed differently associated with the alteration of MDSC . Conclusion Our study provides evidence showing an increased population of L in −/low CD 33 + HLA‐DR − MDSC in the peripheral blood of HCC patients. Our data also suggest that MMP ‐13 and COX ‐2 in PBMC may play a new important role companied with MDSC in HCC patients.