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IL‐9 contributes to the host immune response against Helicobacter pylori and helps limit infection in a Mouse Model
Author(s) -
He Yafei,
Ning Jie,
Li Bin,
Guo Hong,
Hao Ningbo,
Wu Chao
Publication year - 2021
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/hel.12827
Subject(s) - helicobacter pylori , inflammation , immune system , immunology , cytokine , biology , helicobacter pylori infection , interleukin , microbiology and biotechnology , interleukin 10 , proinflammatory cytokine , genetics
Background As an important mediator in lots of diseases, interleukin‐9 (IL‐9) can be a protector or pro‐inflammatory cytokine depending on the complicated inflammatory milieu. Helicobacter pylori ( H . pylori ) induced a series of immunology cells and cytokines change, and however, the role of IL‐9 in H . pylori infection remains unknown. Materials and methods Wild‐type and IL‐9 deficient mice were infected with H . pylori by means of intragastric administration. The colonization of H . pylori bacteria was measured by detecting specific 16s rDNA, and the intensity of inflammation was observed by H&E stain. The expression level of inflammation cytokines was determined by ELISA and quantitative real‐time PCR. Results IL‐9 was increased due to the attack of H . pylori , besides deletion of Il9 aggravated the bacterial colonization and inflammation intensity. In addition, treatment of rmIL‐9 reduced colonized H . pylori and inflammation level, indicated that IL‐9 was a protector for the host against this bacterium. Followed by the H . pylori infection, interferon (IFN)‐γ and interleukin (IL)‐17A were up‐regulated as expected, and nevertheless, the expression of IL‐17A shared a positive relationship with IL‐9 while IFN‐γ negative associated with IL‐9. Moreover, we also proved that Treg cells were not involved in the protective effect of IL‐9, and meanwhile, CD4 + CD25 − T cells secreted more IFN‐γ and less IL‐17A in vitro due to the deletion of Il9 . Conclusions IL‐9 plays a protective role against H . pylori and the protection associated with cytokines change including IFN‐γ and IL‐17A.