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Characteristics of Helicobacter pylori strains isolated from Mauritanian patients
Author(s) -
Khiddi Fatimetou,
Abdellahi Mohamed val Mohamed,
Horma Mohamed Abdallah,
Billoet Annick,
Collobert Ghislaine,
Amar Ahmed Mohamed,
Nech Hachem Demine Moctar,
Vadel El Heiba Mohamed,
Houmeida Ahmed,
Raymond Josette,
Dauga Catherine,
Gastli Nabil
Publication year - 2020
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/hel.12726
Subject(s) - caga , helicobacter pylori , clarithromycin , virulence , biology , microbiology and biotechnology , gene , gastroenterology , medicine , genetics
Background Helicobacter pylori ( H pylori ) is responsible for various diseases including cancer It co‐evolved with humans, and human migrations shaped the expansion and the diversity of strains around the world. The risk of developing a disease depends on virulence factors, mainly the cytotoxin‐associated gene A protein (CagA). The aim of this study was to determine the cagA status in H pylori strains from Mauritanian patients and to search for a relationship with endoscopic and histologic findings. Material and methods H pylori was searched in gastric biopsies taken during endoscopy in patients with gastro‐duodenal symptoms. RT‐PCR was used for the diagnosis and resistance to clarithromycin. The cagA status was determined with PCR and the EPIYA‐cagA polymorphism with sequencing. Results At all, 76/78 (97.4%) biopsies were positive. The rate of clarithromycin resistance was 4/76 (5.26%) due to the A2143G mutation, with a mixed population in 2 cases. The cagA gene was present in 23/76 (30.26%) biopsies, and the EPIYA motif was ABC in 21 (91.3%). High bacterial load and inflammation were significantly associated with cagA ‐positive status ( P < .01). Phylogenetic analysis of the glmM and hspA genes highlighted a mixture of African and European genes in strains of H pylori isolated from patients of Moor origin. Conclusion We report a high prevalence of H pylori infection in Mauritanian patients, a low rate of clarithromycin resistance (5.26%) and high bacterial load and inflammation associated with cagA ‐positive status. The phylogenetic analysis highlights the mix of different populations leading to the Moor ethnicity.