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Interleukin‐27 is abrogated in gastric cancer, but highly expressed in other Helicobacter pylori‐ associated gastroduodenal diseases
Author(s) -
Rocha Gifone A.,
Melo Fabrício F.,
Cabral Mônica M. D. A.,
Brito Breno B.,
Silva Filipe A. F.,
Queiroz Dulciene M. M.
Publication year - 2020
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/hel.12667
Subject(s) - helicobacter pylori , cytokine , peripheral blood mononuclear cell , medicine , gastritis , cancer , immune system , helicobacter , gastric mucosa , interleukin , chronic gastritis , gastroenterology , immunology , stomach , biology , in vitro , biochemistry
Background IL‐27 has dual roles in the immune response either stimulating Th1 or inhibiting Th17 cells. Because there is a particular link of IL‐23/Th17 axis in the development of cancer and IL‐27 has been considered a potential treatment for cancer, we evaluated the gastric and serum concentrations of IL‐27 in two mutually exclusive Helicobacter pylori‐ associated diseases, gastric cancer (GC) and duodenal ulcer (DU). Material and Methods We prospectively studied 110 H pylori ‐positive patients and 40 healthy blood donors. Serum and gastric concentrations of IL‐27 and cytokines of the Th1/Th17 cells were assessed by ELISA. Results IL‐27 was not detected in GC patients, but the cytokine concentration was very high in the patients with DU. IL‐27 was also detected in the gastritis patients and in the H pylori ‐positive blood donors. IL27RA mRNA expression in peripheral blood mononuclear cells, evaluated by rt‐PCR, was stimulated by H pylori strains. The cytokine concentration positively correlated with the Th1 and negatively with Th17 cell representative cytokine levels. Gastric IL‐27 concentrations were positively correlated with increased degree of mononuclear and polymorphonuclear cells on the antral gastric mucosa of DU patients in consonance with the DU gastritis pattern. IL‐12p70 and IFN‐γ gastric concentrations were significantly higher in DU than in GC. Conversely, gastric concentrations of Th17 cell‐associated cytokines (IL‐1β, IL‐6, IL‐17A, IL‐23, and TGF‐β) were significantly higher in GC than in DU patients. Conclusion Although H pylori infection is able to elicit IL‐27 and IL‐27Rα secretion, DU and GC have diametrically opposed cytokine patterns.