Premium
Effective antimicrobial activity of rifabutin against multidrug‐resistant Helicobacter pylori
Author(s) -
Siavoshi Farideh,
Saniee Parastoo,
Malekzadeh Reza
Publication year - 2018
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/hel.12531
Subject(s) - rifabutin , antibiotics , microbiology and biotechnology , clarithromycin , helicobacter pylori , antibiotic resistance , antimicrobial , multiple drug resistance , amoxicillin , agar dilution , metronidazole , biology , medicine , minimum inhibitory concentration
Background Helicobacter pylori resistance to more than one antibiotic is the main reason for failure in bacterial eradication in a considerable number of patients. Rifabutin ( RFB ) with a broad‐spectrum of antimicrobial therapy has been suggested for treatment of refractory multidrug‐resistant infections. Methods Helicobacter pylori isolates from 104 patients were examined for resistance to 5 currently used antibiotics and RFB , using agar dilution method. Twofold serial dilutions of antibiotics were used and MIC s (μg/mL) determined as metronidazole ( MTZ 8), clarithromycin ( CLR 2), amoxicillin ( AMX 1), tetracycline ( TET 0.5), furazolidone ( FRZ 0.5), and RFB (0.06). Results Of 104 H. pylori isolates, only 7 (6.7%) were sensitive to all the 6 antibiotics. However, 30 (28.8%) were resistant to one antibiotic, 28 (26.9%) to two, 19 (18.2%) to three, 14 (13.4%) to four, and 6 (5.7%) to five currently used antibiotics. Overall, 67(64.4%) of isolates were resistant to 2‐5 currently used antibiotics and considered as multidrug‐resistant ( MDR ), with 59 (88.1%) showing sensitivity to RFB and 8 (11.9%) resistance ( P < 0.05). Of 33 isolates resistant to both MTZ and CLR , 25 (75.7%) were sensitive to RFB and 8 (24.3%) resistant ( P < 0.05). Discussion In vitro antimicrobial effectiveness of RFB on MDR H. pylori including those with resistance to both MTZ and CLR was demonstrated. However, RFB efficacy decreased as the number of antibiotics responsible for MDR increased. Considering that RFB inhibits both extra‐ and intracellular H. pylori , it can be suggested as an effective antibiotic against of MDR H. pylori .