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A 21–35 kDa Mixed Protein Component from Helicobacter pylori Activates Mast Cells Effectively in Chronic Spontaneous Urticaria
Author(s) -
Tan RanJing,
Sun HeQiang,
Zhang Wei,
Yuan HanMei,
Li Bin,
Yan HongTao,
Lan ChunHui,
Yang Jun,
Zhao Zhuo,
Wu JinJin,
Wu Chao
Publication year - 2016
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/hel.12312
Subject(s) - helicobacter pylori , histamine , immunoglobulin e , antibody , mast cell , in vitro , protein a , chemistry , immunology , microbiology and biotechnology , biology , medicine , biochemistry , pharmacology
Background Helicobacter pylori ( H. pylori ) seem to involve in the etiology of chronic spontaneous urticaria ( CSU ). But studies of the pathogenic mechanism are very little. Methods In this study, we detected the serum‐specific anti‐ H. pylori IgG and IgE antibodies in 211 CSU and 137 normal subjects by enzyme‐linked immunosorbent assay ( ELISA ), evaluated the direct activation effects of H. pylori preparations and its protein components on human LAD 2 mast cell line in vitro, and analyzed the specific protein ingredients and functions of the most effective H. pylori mixed protein component using liquid chromatography–mass spectrometry and ELISA assay. Results In CSU patients, the positive rate of anti‐ H. pylori IgG positive rate was significantly higher than that in normal controls, and the anti‐ H. pylori IgE levels had no statistical difference between H. pylori ‐infected patients with and without CSU . Further studies suggested that H . pylori preparations can directly activate human LAD 2 mast cell line in a dose‐dependent manner and its most powerful protein component was a mixture of 21–35 kD a proteins. Moreover, the 21–35 kD a mixed protein component mainly contained 23 kinds of proteins, which can stimulate the release of histamine, TNF ‐a, IL ‐3, IFN ‐γ, and LTB 4 by LAD 2 cells in a dose‐dependent or time‐dependent manner. Conclusions A 21–35 kD a mixed protein component should be regarded as the most promising pathogenic factor contributing to the CSU associated with H. pylori infection.