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Helicobacter Species Identified in Captive Sooty Mangabeys ( Cercocebus atys ) with Metastatic Gastric Adenocarcinoma
Author(s) -
Esmail Michael Y.,
Bacon Rebecca,
Swennes Alton G.,
Feng Yan,
Shen Zeli,
Garcia AnaPatricia,
Sharma Prachi,
Cohen Joyce,
Fox James G.
Publication year - 2016
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/hel.12269
Subject(s) - biology , helicobacter , helicobacter pylori , adenocarcinoma , gastric carcinoma , cancer , stomach , pathology , medicine , genetics , biochemistry
Background Of all human cancers, gastric carcinoma is the one of the leading causes of death. Helicobacter pylori is considered a major etiologic agent of this disease. Spontaneously occurring gastric carcinoma is a rare diagnosis in nonhuman primates. A 2011 case report documented a high incidence of gastric adenocarcinoma in a closed colony of captive sooty mangabeys ( Cercebus atys ). However, H. pylori infection was not detected in these animals. Materials and Methods In this study, using archived formalin‐fixed, paraffin‐embedded stomach sections of these animals alternative methodologies were used to identify H. pylori and other non‐ H. pylori Helicobacter species. In addition, two additional cases of sooty mangabeys with metastatic gastric carcinoma are characterized. Results Using fluorescent in situ hybridization, we identified gastric H. suis in 75% of archived and new gastric carcinoma cases. In the two newly reported cases, H. suis and a novel Helicobacter species were detected via PCR and sequence analysis of the 16S rRNA gene. H. pylori was not identified in any of the gastric carcinoma cases via FISH and/or PCR and sequence analysis of Helicobacter spp. in DNA from of available tissues. Conclusions This report is the first to characterize Helicobacter species infection in spontaneous gastric carcinoma with metastatic potential in nonhuman primates.