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Mouse Models for Assessing the Protective Efficacy of Lactobacillus gasseri SBT 2055 against Helicobacter suis Infection Associated with the Development of Gastric Mucosa‐Associated Lymphoid Tissue Lymphoma
Author(s) -
Matsui Hidenori,
Takahashi Tetsufumi,
Øverby Anders,
Murayama Somay Yamagata,
Yoshida Haruno,
Yamamoto Yuji,
Nishiyama Keita,
Seto Yasuyuki,
Takahashi Takashi,
Mukai Takao,
Nakamura Masahiko
Publication year - 2015
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/hel.12203
Subject(s) - microbiology and biotechnology , biology , lymphatic system , lactobacillus gasseri , cancer , lactobacillus , immunology , medicine , bacteria , genetics
Background Helicobacter suis strain TKY infection has been strongly associated with the development of gastric mucosa‐associated lymphoid tissue ( MALT ) lymphoma in a C57 BL /6J mouse model. Materials and Methods 1. C57 BL /6J mice were intragastrically administered Lactobacillus strains once daily with 10 8 –10 9 colony‐forming units ( CFU ), starting 2 days before intragastric infection with H. suis TKY (approximately 1 × 10 4 copies of 16S rRNA genes) or H. pylori Sydney strain 1 ( SS 1; 3 × 10 8 CFU ) and continuing for 14 days after infection. 2. C57 BL /6J mice were given powdered feed mixed with lyophilized L. gasseri SBT 2055 ( LG 2055) cells (5 × 10 8 CFU /g), starting 2 weeks before intragastric infection with H. suis TKY and continuing 12 months after infection. Results 1. Among the 5 Lactobacillus strains that we examined, only LG 2055 exhibited significantly preventive efficacy against both H. suis TKY and H. pylori SS 1 at day 15 after infection. 2. Dietary supplementation with LG 2055 protected mice from the formation of round protrusive lesions in the gastric fundus 12 months after infection with H. suis TKY , whereas such lesions had developed in the gastric fundus of nonsupplemented mice 12 months after infection. In addition, the formation of lymphoid follicles in gastric mucus layers was suppressed by dietary LG 2055 at 3 months after infection. Conclusions LG 2055 administration is effective for suppressing the progression of gastric MALT lymphoma by reducing H. suis colonization.