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IL 10 Single Nucleotide Polymorphisms are Related to Upregulation of Constitutive IL ‐10 Production and Susceptibility to Helicobacter pylori Infection
Author(s) -
Assis Shirleide,
Marques Cintia Rodrigues,
Silva Thiago Magalhães,
Costa Ryan Santos,
AlcantaraNeves Neuza Maria,
Barreto Mauricio Lima,
Barnes Kathleen Carole,
Figueiredo Camila Alexandrina
Publication year - 2014
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/hel.12119
Subject(s) - helicobacter pylori , single nucleotide polymorphism , immunology , biology , helicobacter , interleukin 10 , genotype , genetics , immune system , gene
Background Helicobacter pylori infection is a strong risk factor for gastric cancer, likely due to the extensive inflammation in the stomach mucosa caused by these bacteria. Many studies have reported an association between IL 10 polymorphisms, the risk of gastric cancer, and IL ‐10 production. The aim of the study was to evaluate the association between IL 10 genetic variants, Helicobacter pylori infection, and IL ‐10 production by peripheral blood leukocytes in children. Materials and Methods We genotyped a total of 12 single nucleotide polymorphisms in IL 10 in 1259 children aged 4–11 years living in a poor urban area in Salvador, Brazil, using TaqMan probe based, 5′ nuclease assay minor groove binder chemistry. Association tests were performed by logistic regression for Helicobacter pylori infection and linear regression for IL ‐10 spontaneous production (whole‐blood cultures) including sex, age, and principal components for informative ancestry markers as covariates, using PLINK . Results Our results shown that IL 10 single nucleotide polymorphisms rs1800896 ( OR = 1.63; 95% CI = 1.11–2.39), rs3024491 ( OR = 1.71; 95% CI = 1.14–2.57), rs1878672 ( OR = 1.79; 95% CI = 1.19–2.68), and rs3024496 ( OR = 1.48; 95% CI = 1.05–2.08) were positively associated with Helicobacter pylori infection. Eight single nucleotide polymorphisms were associated with spontaneous production of IL ‐10 in culture, of which three (rs1800896 and rs1878672, p = .04; rs3024491, p = .01) were strongly associated with infection by Helicobacter pylori . Conclusions Our results indicate that IL 10 variants rs1800896, rs3024491, rs1878672, and rs3024496 are more consistently associated with the presence of anti‐ H. pylori IgG by inducing increased production of IL ‐10. Further studies are underway to elucidate the role of additional genetic variants and to investigate their impact on the occurrence of gastric cancer.