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Helicobacter pylori Susceptible/Resistant to Antibiotic Eradication Therapy Differ in the Maturation and Activation of Dendritic Cells
Author(s) -
Kopitar Andreja N.,
Skvarc Miha,
Tepes Bojan,
Kos Janko,
Ihan Alojz
Publication year - 2013
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/hel.12068
Subject(s) - helicobacter pylori , microbiology and biotechnology , biology , secretion , helicobacter , immune system , immunology , antigen , biochemistry , genetics
Background The natural course of H elicobacter pylori infection, as well as the success of antibiotic eradication is determined by the immune response to bacteria. The aim of the study is to investigate how different H elicobacter pylori isolates influence the dendritic cells maturation and antigen‐presenting function in order to elucidate the differences between H elicobacter pylori strains, isolated from the patients with successful antibiotic eradication therapy or repeated eradication failure. Materials and Methods Dendritic cells maturation and antigen presentation were monitored by flow cytometry analysis of the major histocompatibility complex class II ( MHC ‐II), Toll‐like receptor ( TLR ) and costimulatory molecules expression, and by determining cytokine secretion. Results Dendritic cells stimulated with H elicobacter pylori isolated from patients with repeated antibiotic eradication failure expressed less human leukocyte antigen ( HLA‐DR ), CD 86, TLR ‐2, and interleukin‐8 ( IL ‐8) compared to H elicobacter pylori strains susceptible to antibiotic therapy; the latter expressed lower production of IL ‐10. Polymyxin B inhibition of lipopolysaccharide reduces IL ‐8 secretion in the group of H elicobacter pylori strains susceptible to antibiotic therapy. The differences in IL ‐8 secretion between both groups are lipopolysaccharide dependent, while the differences in secretion of IL ‐10 remain unchanged after lipopolysaccharide inhibition. Inhibitor of cathepsin X Mab 2F12 reduced the secretion of IL ‐6, and the secretion was significantly lower in the group of H elicobacter pylori strains isolated from patients with repeated antibiotic eradication failure. Conclusion Helicobacter pylori strains, susceptible/resistant to antibiotic eradication therapy, differ in their capability to induce DC s maturation and antigen‐presenting function.

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