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Replication of Migraine GWAS Susceptibility Loci in C hinese H an Population
Author(s) -
Fan Xiaoping,
Wang Jing,
Fan Wen,
Chen Lixue,
Gui Bei,
Tan Ge,
Zhou Jiying
Publication year - 2014
Publication title -
headache: the journal of head and face pain
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.14
H-Index - 119
eISSN - 1526-4610
pISSN - 0017-8748
DOI - 10.1111/head.12329
Subject(s) - genome wide association study , single nucleotide polymorphism , genotyping , migraine with aura , odds ratio , migraine , genetics , genetic association , genotype , allele , aura , population , snp , biology , medicine , gene , environmental health
Background Recent genome‐wide association studies ( GWAS ) have identified 3 genetic variants that are strongly associated with migraine in E uropeans. The effect of these risk variants in other populations is unknown. To further replicate the GWAS findings, we investigated the 3 variants rs2651899 (1p36.32, PRDM 16), rs10166942 (2q37.1, TRPM 8), and rs11172113 (12q13.3, LRP 1) for their association with migraine in the C hinese H an population. Methods We performed a case–control association study. Genomic DNA was collected from 608 unrelated individuals, including 304 migraineurs (41 migraine with aura and 263 migraine without aura) and 304 healthy controls. Genotyping of single nucleotide polymorphisms ( SNPs ) was performed by ligase detection reaction method. Results We identified the minor allele of rs2651899 located in PRDM 16 to be associated with migraine ( P = .005, odds ratio = 1.382, 95% confidence interval = 1.100‐1.736), the association remain significant after B onferroni correction. For the other 2 SNPs (rs10166942 and rs11172113), no statistically significant differences were observed in the allele/genotype frequencies between cases and controls. None of the 3 SNP was associated with specific migraine features. Conclusion Our study confirmed the association of PRDM 16 to migraine susceptibility in the C hinese H an population. The results also indicated that replication studies of previous GWAS findings across populations is of importance to validate these associations and to gain a better understanding of migraine susceptibility of potential genetic heterogeneity between populations. Further work is necessary to understand the functional mechanisms underlying these variants identified by GWAS .