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Positive association between plasma levels of oxidized low‐density lipoprotein and myeloperoxidase after hemodialysis in patients with diabetic end‐stage renal disease
Author(s) -
Kitabayashi Chizuko,
Naruko Takahiko,
Sugioka Kenichi,
Yunoki Kei,
Nakagawa Masashi,
Inaba Mayumi,
Ohsawa Masahiko,
Konishi Yoshio,
Imanishi Masahito,
Inoue Takeshi,
Itabe Hiroyuki,
Yoshiyama Minoru,
Haze Kazuo,
Becker Anton E.,
Ueda Makiko
Publication year - 2013
Publication title -
hemodialysis international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.658
H-Index - 47
eISSN - 1542-4758
pISSN - 1492-7535
DOI - 10.1111/hdi.12049
Subject(s) - medicine , hemodialysis , end stage renal disease , myeloperoxidase , low density lipoprotein , diabetes mellitus , lipoprotein , plasma levels , gastroenterology , endocrinology , cholesterol , inflammation
End‐stage renal disease ( ESRD ) patients undergoing hemodialysis ( HD ) have a high prevalence of cardiovascular events. Low‐density lipoprotein ( LDL ) in dialysis patients has been shown to be susceptible to in vitro peroxidation; therefore, oxidized‐ LDL (ox‐ LDL ) could be generated in these patients. Moreover, myeloperoxidase ( MPO ) released from activated neutrophils may play a role in the induction of LDL oxidation. The purpose of this study was to investigate the relationship between plasma ox‐ LDL levels, plasma MPO levels, and serum high‐sensitivity C ‐reactive protein (hs‐ CRP ) levels during initial HD in patients with diabetic ESRD . Patients ( n = 28) had serial venous blood samples drawn before and after HD at the initial, second, and third sessions. Plasma ox‐ LDL levels were measured using a specific monoclonal antibody ( DLH3 ), and plasma MPO levels were measured using an enzyme‐linked immunosorbent assay kit. Plasma ox‐ LDL levels and MPO levels after a single HD session increased significantly (ox‐ LDL , P < 0.005; MPO , P < 0.0001) compared with levels before that HD session. However, the increase was transient since the levels returned to pre‐ HD session levels. Additionally, plasma MPO levels showed a positive correlation with plasma ox‐ LDL levels during HD (R = 0.62, P = 0.0029). No significant change was observed in serum hs‐ CRP levels before and after each HD session. This study demonstrates that plasma MPO levels are directly associated with plasma ox‐ LDL levels in diabetic ESRD patients during initial HD . These findings suggest a pivotal role for MPO and ox‐ LDL in the progression and acceleration of atherosclerosis in patients undergoing HD .