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Comparison of von Willebrand factor ( VWF ) activity levels determined by Hemos IL AcuStar assay and Hemos IL LIA assay with ristocetin cofactor assay by aggregometry
Author(s) -
Sagheer S.,
Rodgers S.,
Yacoub O.,
Dauer R.,
Mcrae S.,
Duncan E.
Publication year - 2016
Publication title -
haemophilia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.213
H-Index - 92
eISSN - 1365-2516
pISSN - 1351-8216
DOI - 10.1111/hae.12937
Subject(s) - ristocetin , von willebrand factor , von willebrand disease , immunoassay , medicine , immunology , microbiology and biotechnology , chemistry , platelet , antibody , biology
Diagnosis of von Willebrand disease ( VWD ) requires quantitative as well as qualitative determination of von Willebrand factor ( VWF ) levels. For functional assessment of VWF , ristocetin cofactor assay by aggregometry is considered to be the gold standard. However, need for technical expertise, labour intensiveness, difficult standardization and high intra‐ and inter‐ assay variabilities are some of the limitations of this methodology. Various assays for determination of VWF adhesive function using different methodologies have been developed in recent years. Aim To evaluate the Hemos IL AcuStar chemiluminescence assay ( VWF : RC o[Acu]) and the Hemos IL latex immunoassay ( VWF :act) as diagnostic tests for VWD and identification of type 2 VWD in comparison with the ristocetin cofactor assay performed by aggregometry ( VWF : RC o[Agg]). Methods Results from 96 samples analysed by VWF : RC o[Acu] and 128 samples by VWF :act were compared with VWF : RC o[Agg]. Sixty of these samples (25 normal, 17 type 1 and 18 type 2) were analysed by all three assays. Results VWF : RC o[Acu] showed excellent agreement with VWF : RC o[Agg], and readily identified all type 2 VWD samples tested. VWF :act showed reasonable agreement with VWF : RC o[Agg] for most patients, but had a slightly lower sensitivity for detection of type 2 VWD . Conclusion VWF : RC o[Acu] assay has the potential to replace VWF : RC o[Agg] for the diagnosis of VWD .