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Ubiquitination‐dependent and ‐independent repression of target genes by SETDB1 reveal a context‐dependent role for its methyltransferase activity during adipogenesis
Author(s) -
Zhang Ji,
Matsumura Yoshihiro,
Kano Yuka,
Yoshida Ayano,
Kawamura Takeshi,
Hirakawa Hiroyuki,
Inagaki Takeshi,
Tanaka Toshiya,
Kimura Hiroshi,
Yanagi Shigeru,
Fukami Kiyoko,
Doi Takefumi,
Osborne Timothy F.,
Kodama Tatsuhiko,
Aburatani Hiroyuki,
Sakai Juro
Publication year - 2021
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12868
Subject(s) - biology , histone methyltransferase , chromatin , histone methylation , adipogenesis , histone , gene silencing , heterochromatin protein 1 , methyltransferase , epigenetics , histone h3 , microbiology and biotechnology , dna methylation , gene , genetics , methylation , heterochromatin , gene expression
The lysine methyltransferase SETDB1, an enzyme responsible for methylation of histone H3 at lysine 9, plays a key role in H3K9 tri‐methylation‐dependent silencing of endogenous retroviruses and developmental genes. Recent studies have shown that ubiquitination of human SETDB1 complements its catalytic activity and the silencing of endogenous retroviruses in human embryonic stem cells. However, it is not known whether SETDB1 ubiquitination is essential for its other major role in epigenetic silencing of developmental gene programs. We previously showed that SETDB1 contributes to the formation of H3K4/H3K9me3 bivalent chromatin domains that keep adipogenic Cebpa and Pparg genes in a poised state for activation and restricts the differentiation potential of pre‐adipocytes. Here, we show that ubiquitin‐resistant K885A mutant of SETDB1 represses adipogenic genes and inhibits pre‐adipocyte differentiation similar to wild‐type SETDB1. We show this was due to a compensation mechanism for H3K9me3 chromatin modifications on the Cebpa locus by other H3K9 methyltransferases Suv39H1 and Suv39H2. In contrast, the K885A mutant did not repress other SETDB1 target genes such as Tril and Gas6 suggesting SETDB1 represses its target genes by two mechanisms; one that requires its ubiquitination and another that still requires SETDB1 but not its enzyme activity.