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Multiple nuclear localization sequences in SRSF4 protein
Author(s) -
Masaki So,
Kabuto Takafumi,
Suzuki Kenji,
Kataoka Naoyuki
Publication year - 2020
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12756
Subject(s) - nuclear localization sequence , nls , biology , rna splicing , subcellular localization , nuclear transport , nuclear protein , alternative splicing , fusion protein , arginine , microbiology and biotechnology , serine , protein domain , sr protein , cell nucleus , protein subcellular localization prediction , nucleus , computational biology , amino acid , phosphorylation , genetics , gene , cytoplasm , transcription factor , gene isoform , recombinant dna , rna
SRSF4 is one of the members of serine‐/arginine (SR)‐rich protein family involved in both constitutive and alternative splicing. SRSF4 is localized in the nucleus with speckled pattern, but its nuclear localization signal was not determined. Here, we have identified nuclear localization signals (NLSs) of SRSF4 by using a pyruvate kinase fusion system. As expected, arginine‐/serine (RS)‐rich domain of SRSF4 confers nuclear localization activity when it is fused to PK protein. We then further delineated the minimum sequences for nuclear localization in RS domain of SRSF4. Surprisingly, RS‐rich region does not always have a nuclear localization activity. In addition, basic amino acid stretches that resemble to classical‐type NLSs were identified. These results strongly suggest that SRSF4 protein uses two different nuclear import pathways with multiple NLSs in RS domain.