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Forkhead box B2 inhibits the malignant characteristics of the pancreatic cancer cell line Panc‐1 in vitro
Author(s) -
Fukuchi Hiroki,
Hayashida Yukinobu,
Inoue Kunio,
Sadamura Yoshifusa
Publication year - 2019
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12717
Subject(s) - biology , in vitro , pancreatic cancer , cancer research , cell culture , line (geometry) , cancer , cancer cell lines , microbiology and biotechnology , computational biology , cancer cell , biochemistry , genetics , geometry , mathematics
Abstract Forkhead box (FOX) proteins constitute a family of transcription factors that are evolutionarily conserved in various species ranging from yeast to humans. These proteins have functions during development as well as in adulthood. To date, many reports have described the functions of FOX family genes in cancer cells, but the role of FOXB2 is not well understood. In one of the pancreas ductal adenocarcinoma cell lines, Panc‐1 cells, we showed here that FOXB2 expression is barely detectable and that CpG islands in the 5′ regions of the FOXB2 are highly methylated. These findings led us to hypothesize that FOXB2 acts as a tumor suppressor. To clarify our hypotheses, we investigated the effects of FOXB2 over‐expression in Panc‐1 cells. We obtained FOXB2 stable transfectants, and these clones exhibited reduced spheroid formation ability. Expression of β‐catenin, which is reported to be over‐expressed in various cancer cells, was highly suppressed in FOXB2 stable transfectants. Moreover, side population (SP) cell fractions, which have a high tumorigenesis and metastatic potential, as well as anchorage‐independent growth ability, were reduced. These results suggest that FOXB2 has the ability to inhibit the malignant characteristics of Panc‐1 in vitro.