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Enhancer role of a native metabolite, O ‐acetyl‐ADP‐ribose, on the Saccharomyces cerevisiae chromatin epigenetic gene silencing
Author(s) -
Wang SueHong,
Tung ShuYun,
Su KuanChung,
Shen HsiaoHsuian,
Hong JiaYang,
Tsai MingShiun,
Liou GunnGuang
Publication year - 2019
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12685
Subject(s) - biology , heterochromatin , chromatin , gene silencing , epigenetics , histone , saccharomyces cerevisiae , nucleosome , microbiology and biotechnology , genetics , gene
To study the epigenetic gene silencing, yeast is an excellent model organism. Sir proteins are required for the formation of silent heterochromatin. Sir2 couples histone deacetylation and NAD hydrolysis to generate an endogenous epigenetic metabolic small molecule, O ‐acetyl‐ADP‐ribose (AAR). AAR is involved in the conformational change of SIR complexes, modulates the formation of SIR–nucleosome preheterochromatin and contributes to the spreading of SIR complexes along the chromatin fiber to form extended silent heterochromatin regions. Here, we show that AAR is capable of enhancing the chromatin silencing effect under either an extra exogenous AAR or a defect AAR metabolic enzyme situation, but decreasing the chromatin silencing effect under a defect AAR synthetic enzyme state. Our results provide an evidence of biological function importance of AAR. It is indicated that AAR does not only function in vitro but also play a role in vivo to increase the effect of heterochromatin epigenetic gene silencing. However, further investigations of AAR are warranted to expand our knowledge of epigenetics and associated small molecules.